| Cat.No. | Size | Price(US$) | |
| EUT-500 | 500U | 12.00 | |
Description The Polymerase gene from Thermus aquaticus (strain YT1) is cloned and expressed in E. coli, then highly purified to produce U-Taq DNA polymerase. It is used in the amplification and sequence testing of DNA through PCR. The quality of the U-Taq DNA polymerase has been tested by the Activity Test, SDS-Page, Endonuclease Test, etc. Reaction Buffer (10X): 100 mM Tris-HCl, 400mM KCI, 15mM MgCl2 , pH9.0 Dilution Buffer: 20mL Tris-HCl, 100mM KCI, 0.5 mM EDTA, 1mM DTT, 0.5% Tween 20, 0.5 % IGEPAL CA-630(pH 8.0) Storage Buffer: 20 mM Tris-HCI, 0.5 mM EDTA, 1mM DTT, 0,5% Tween 20, 0.5% IGEPAL CA-630(pH 8.0.). Store at -20℃. Concentration: 5,000 units/mL Involve the dNTPs mixture: The concentration of each dNTPs is 2.5 mM. |
|
 |
Description |
● This red dye enables quick visual
confirmation of enzyme addition and
reaction mixing, which makes performance
more convenient.
●After PCR
amplification, samples can be removed
from the reaction and loaded directly
onto agarose gel without the
addition of loading buffer or tracking
dye. The red dye, acting as a tracking
dye, migrates between bromophenol blue
and xylenecyanol at about the
same rate as 400~500bp fragment.
● The concentration of Taq-red is only
1unit/µl, so the enzyme can be
transferred and added more accurately.
● The enzyme generates PCR products with
3'-dA overhangs, suitable for T-A
cloning.
Cat.No. |
Size |
Price(US$) |
EP-500 |
500U |
27.00 |
Description
s-Pfu DNA Polymerase is a thermostable enzyme with a
molecular weight of 90 kDa. It catalyzes the
polymerization of nucleotides into duplex DNA in the 5'→3' direction, resulting in blunt-ended PCR products
without 3'-dA overhangs.
s-Pfu DNA Polymerase exhibits 3'→5' exonuclease (proofreading) activity that enables
the polymerase to correct the mis-incorporation of
nucleotide, and lacks 5'→3' exonuclease activity. It is
suitable for PCR and primer extension reaction that
requires high fidelity when the PCR fragment is
relatively shorter
than 3kb.
The extension rate of s-Pfu DNA Polymerase is about
600bp/min in standard condition. The appropriate
reaction temperature is 65℃~75℃, the working concentration
of dNTPs is 100-300μM, the working concentration of Mg2+ is
2~3mM, and the optimal pH is 8.1~9.1. The amount of
enzyme is 1~1.5unit for 20μl PCR reaction, while
2~3units for 50μl PCR reactio
Cat.No. |
Size |
Price(US$) |
EVT-500 |
500U |
24.00 |
Description
Taq DNA Polymerase is the most common PCR enzyme for
amplifying up to 10kb Lambda DNA templates and up to 3kb
genomic templates. However, it is not able to amplify
even longer DNA fragments. To overcome this constraint,
variable PCR systems have been developed by various
companies, which are containing Taq DNA Polymerase and
thermostable DNA Polymerase with proofreading activity.
V-Taq DNA Polymerase is the PCR System which are
developed for amplifying long and complex fragments. It
can amplify longer fragments up to 20kb from human
genomic DNA, and up to 30kb from a Lambda DNA template).
Moreover, it has improved amplification efficiency
compared to Taq DNA Polymerase by improving enzyme
activity. Therefore V-Taq DNA Polymerase is a more
versatile enzyme blend in amplifying various templates
including short and long DNA fragment or simple and
complex DNA, either.
| Desription | Cat.No. | Size | Price(US$) | |
| 0.2 ml thin-well tube, 20 μl reaction | EQ2.2-50 | 50 tubes | 25.00 | |
| 0.2 ml thin-well tube, 50 μl reaction | EQ5.2-50 | 50 tubes | 37.00 | |
| 0.5 ml thin-well tube, 20 μl reaction | EQ2.5-50 | 50 tubes | 25.00 | |
| 0.5 ml thin-well tube, 50 μl reaction | EQ5.5-50 | 50 tubes | 37.00 | |
Description:
The Easy-DoTM PCR PreMix is a pre-mixed preparation in lyophilized format. The mix contains U-Taq DNA polymerase,
reaction buffer, dNTPs, loading buffer and tracking dye for efficient PCR amplification. For reaction set-up, all you have
to do is to add templates, specific primers and water. After PCR amplification, samples can be loaded directly onto agarose
gel without addition of loading buffer or tracking dye. The Easy-DoTM PCR premix simplifies the assembly of PCR reaction
and offers advantages of time savings, convenience, consistency, and minimal risk of contamination.
Storage:store at -20oC for one year.
Cat.No. |
Size |
Price(US$) |
EN-1 |
1ml |
14.00 |
Description:
10 mM dNTPs Mix is a ready-to-use solution of dATP, dCTP, dGTP and dTTP (monosodium salts) at a concentration of
10 mM each in sterile deionized water at pH7.5, whose purity is ≥99% (HPLC). It is free of RNase and DNase, and is
suitable for any molecular biology application that requires pure deoxynucleotides, such as PCR, DNA sequencing, cDNA
synthesis and nick translation.
Storage: Store at -20℃.
Cat.No. |
Size |
Price(US$) |
EN-2 |
4×0.4ml |
55.00 |
Description:
dNTPs Set (100mM each) contains 4×0.4 ml of dATP, dCTP, dGTP and dTTP (monosodium salts) at a concentration of 100 mM each in sterile deionized water at pH7.5, whose purity is ≥99% (HPLC). It is free of RNase and DNase, and suitable for any molecular biology application that requires pure deoxynucleotides, such as PCR, DNA sequencing, cDNA synthesis and nick translation.
Storage: Store at -20℃.
Cat.No. |
Size |
Price(US$) |
EO-1 |
1ml |
14.00 |
GC-rich regions may form rigid secondary structure which makes difficult or impossible for the DNA polymerases to
work under standard PCR conditions. The PCR optimizer can optimize PCR of problematic or GC-rich templates. The
recommended amount is 1/5 of reaction volume.