Custom Oligonucleotides
        SBS has synthesized millions of high-quality oligonucleotides for researchers since it was founded in 1994. The oligos have been applied in almost all fields of molecular biology, and have become the top choice for researchers.
        To meet the increasing market demand, we have introduced the most advanced high-throughput synthesizers, which provide computer-controlled oligo synthesis, cleavage, and deprotection in a parallel-processing format. Our oligos are synthesized with a typical coupling efficiency of 99% and purified by OPC free of charge. PAGE and HPLC purification are also available with additional charge. The high coupling efficiency of our synthesis technology allows efficient synthesis of oligos up to 100 bases. In addition, a variety of modifications are available. Every oligo is analyzed by PAGE to check oligo quality. Malidi-Tof Mass QC is also employed to examine the oligos randomly at our facility. Now we are one of the leading companies in DNA synthesis in China.

Notes: All of the yields indicated below are after OPC, PAGE or HPLC purification.

Standard Oligos
   
Yield Price(US$/base) Working days
2 OD 0.20 2~3days
5 OD 0.23
10 OD 0.28
30 OD 0.66
60 OD 1.05
100 OD 1.87
The price is only for oligos shorter than 60 mer.
Long Oligos
Length Yield Yield Price (US$/base)
60~100 mer   2 OD 0.50
5 OD 1.00
10 OD 1.80
30 OD 3.60
50 OD 4.50
Turn-around time is about 4 working days.
Phosphorothioate Oligos (S-Oligo)
   
5 OD
(US$/bond)
10 OD
(US$/bond)
30 OD
(US$/bond)
60 OD
(US$/bond)
100 OD
(US$/bond)
0.90 1.50 3.00 3.80 6.00
Turn-around time is about four working days.
Modified Oligos

Modification

2 OD (US$)

5 OD (US$)

10 OD (US$)

dInosine

10.50

12.50

20.00

5-Methyl dC

12.50

20.00

30.00

dUridine

12.50

20.00

30.00

O4-Me-dT

17.50

25.50

35.00

5'-Amino Linker C6

17.50

25.50

35.00

5'-Phosphorylation

25.50

32.50

45.00

5'-Biotin

25.50

32.50

45.00

5'-Thiol Modifier C6 S-S

52.50

77.50

112.50

3'-dInosine

10.50

12.50

20.00

3'-Amino Linker C7

32.50

45.00

65.00

3'-Phosphorylation

35.00

52.50

80.00

3'-Biotin

40.00

45.00

65.00

3'-Thiol Modifier C6 S-S

40.00

60.00

82.50

For long oligos (60~100 mer) multiply 1.4 based on the above prices
Turn-around time is about 3-4 working days.
Other modifications are available upon request.
Fluorescent Oligos

Fluorophores

2 OD (US$)

5 OD (US$)

10 OD (US$)

5'-FAM

40.00

45.00

65.00

5'-HEX

65.00

80.00

110.00

5'-TET

65.00

80.00

110.00

5'-CY3

130.00

160.00

220.00

5'-CY5

130.00

160.00

220.00

5'-TAMRA

65.00

80.00

110.00

3'-Fluorescein

40.00

45.00

65.00

3'-TAMRA

65.00

80.00

110.00

3'-Cy3

130.00

160.00

270.00

3'-Cy5

130.00

160.00

270.00

3'-Dabcyl

65.00

80.00

110.00

3'-ROX

80.00

90.00

115.00

3'-BHQ-1

80.00

90.00

115.00

3'-BHQ-2

80.00

90.00

115.00

3'-BHQ-3

80.00

90.00

115.00

For long oligos (60~100 mer) multiply 1.4 based on the above prices
Turn-around time is about 3-4 working days.
Other labelings are available upon request.
Oligo Purification
  OPC PAGE (US$/oligo) HPLC (US$/oligo)
2 OD Free 10.00 10.00
5 OD Free 15.00 15.00
10 OD Free 30.00 30.00
30 OD Free 75.00 75.00
50 OD Free 90.00 90.00
100 OD Free 120.00 120.00
OPC purification is only for < 60 mer standard oligos and S-oligos.
Long oligos (≥ 60 mer) should be PAGE purified.
Modified oligos should be PAGE or HPLC purified.
Probes for Quantitative, Real-time PCR

Taqman

        Taqman probes have a fluorescent reporter and a quencher at their 5' and 3' ends, respectively. These probes can be used in quantitative PCR systems that take advantage of the 5'-3' exonulease activity of Taq DNA polymerase. A probe specific for the sequence of interest is used in PCR together with specific PCR primers. This probe is designed to anneal between the PCR primers. During the extension phase of PCR, the 5'-3' exonulease activity of Taq DNA polymerase cleaves the fluorescent reporter from the probe. The amount of free reporter accumulates as the number of PCR cycles increases. The fluorescent signal from the free reporter is measured in real time and allows quantification of the amount of target sequence.

        SBS Genetech offers a large number of fluorescent reporters and different quenchers for Taqman probes. Taqman probes with Black Hole Quenchers or a TAMRA quencher and a variety of different fluorescent reporters can be conveniently ordered from SBS Genetech. All Taqman probes are provided PAGE or HPLC purified.

Taqman probes 2 OD (US$) 5 OD (US$) 10 OD (US$)
Taqman probes with a Black Hole Quencher
5'FAM and 3'BHQ-1 105.00 125.00 165.00
5' HEX and 3' BHQ-1 135.00 160.00 215.00
5' TET and 3' BHQ-1 135.00 160.00 215.00
5' Cy3 and 3' BHQ-2 185.00 225.00 345.00
5' Cy5 and 3' BHQ-2 185.00 225.00 345.00
5' TAMRA and 3' BHQ-2 120.00 145.00 200.00
5’-CAL Fluor Red 610 and 3’ BHQ-2 175.00 210.00 320.00
5’-Quasar 670 and 3’ BHQ-2 185.00 225.00 345.00
5’-Quasar 605 and 3’ BHQ-2 205.00 250.00 380.00
Taqman probes with a TAMRA Quencher
5' FAM and 3' TAMRA 95.00 115.00 160.00
5' HEX and 3' TAMRA 120.00 145.00 200.00
5' TET and 3' TAMRA 120.00 145.00 200.00
OPC For long oligos (60~100 mer) multiply 1.4 based on the above prices
Turn-around time is about 3-4 working days

Molecular Beacons

        Molecular Beacons also have a fluorescent reporter and a quencher at their 5' and 3' ends, respectively. The sequence of these probes is designed so that they form a hairpin structure in which the fluorescent dye and the quencher are in close proximity. A Molecular Beacon specific for the sequence of interest is used in PCR. The probe is designed to anneal between the PCR primers. When the probe hybridizes to its target sequence in the PCR annealing step, the loop opens and the fluorescent reporter and quencher are separated, resulting in a fluorescent signal. The amount of signal is proportional to the amount of target sequence, and is measured in real time to allow quantification of the amount of target sequence.

        SBS Genetech is a licensed supplier of Molecular Beacons, and offers a large number of fluorescent reporters and different quenchers. Molecular Beacons with a dabcyl quencher and a variety of different fluorescent reporters can be conveniently ordered from SBS Genetech. All Molecular Beacons are provided PAGE or HPLC purified.

Molecular Beacon

2 OD (US$)

5 OD (US$)

10 OD (US$)

5'-FAM and 3' Dabcyl

95.00

115.00

160.00

5'-HEX and 3' Dabcyl

120.00

145.00

200.00

5'-TET and 3' Dabcyl

120.00

145.00

200.00

5'-CY3 and 3' Dabcyl

175.00

215.00

335.00

5'-CY5 and 3' Dabcyl 175.00 215.00 335.00
5'-TAMRA and 3' Dabcyl 105.00 135.00 195.00