SiMax™ PCR Products/Agarose Gel Purification
The SiMax™ PCR Products/Agarose Gel Purification Kit is designed for the rapid purification of PCR products, or for the efficient extraction of DNA fragments from agarose gel. In a high-salt buffer, DNA is bound to the SiMax™ membrane in a spin column. Following a wash step, DNA is eluted in low-salt buffer or water without alcohol precipitation or desalting. This kit removes DNA polymerase, dNTPs, and salts. DNA fragments of 50 bp to 20 kb can be cleaned up within 20 minutes.
Known amount (2 μg) of seven DNA fragments was extracted from the agarose gel using SiMax™ PCR Products/Agarose Gel Purification Kit. 1/10 amount of each recovered fragment was analyzed by agarose gel electrophoresis. Recovery rate was up to 80%.
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