Thermo-stable M-MLV Reverse Transcriptase (RNase H-)



Cat. No.

Concentration

Size

Price (US$)

FMM-10K

200U/μl

10,000U

45.00



Product Description:


MMLV Reverse Transcriptase is able to synthesize the complementary DNA strand from a single-strand RNA template. M-MLV Reverse Transcriptase (RNase H-) is a mutant type of M-MLV Reverse Transcriptase, obtained by eliminating the active center of RNase H through multiple point mutations. The alteration decreases activity of RNase H and reduces RNA degradation in reverse transcription, which increases the yield of first-strand cDNA to get full length cDNA more easily. In addition, thermal stability of the reverse transcriptase is improved and the optimal reaction temperature is therefore 50℃.


Source and Application:


The enzyme is obtained from E.coli strain containing mutant Molony murine leukemia virus (M-MLV) reverse transcriptase gene.It could be applied in first-strand cDNA synthesis and RT-PCR


Product Detail:


Unit Definition

One activity unit (U) refers to the amount of M-MLV reverse transcriptase when catalyzes the incorporation of 1nmol of dTTP into materials in 10 minat 37℃ using oligo (dT) primed Poly (A) as a template

Storage Buffer

20 mMTris-HCl (pH7.5), 200 mMNaCl, 0.25 mM EDTA, 0.01% NP-40 (v/v), 2.5 mM DTT, 50% Glycerol (v/v)

Product Purity

No macroscopic differences on electrophoretic bands after incubatingthe mixture of 200U M-MLV Reverse Transcriptase (RNase H-) and 1μg 16S, 23S rRNA at 37℃ for 1 h

Reaction Buffer

5 x RT Buffer:
250 mM KCl; 15 mM MgCl2; 10 mM DTT; 100 mM Tris-HCl pH 8.4

Storage Condition

-20℃, three years




M-MLV User's Instruction







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