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PrimeTaq™ HotStart Direct PCR DNA Polymerase


Cat. No.

Size

Price (US$)

PTHSD-1k

1KU

280.00

PTHSD-200k

200KU

35000.00

 

For direct amplification of RNA, please see PrimeTaq™ HS DP DNA/RNA Polymerase.

Advantages

  • lRapid amplification: The amplification rate of the polymerase is 6kb/min. 1kb fragment can be amplified within 25min.

  • lLong fragment amplification: For plasmid, λ DNA and other easy templates, the polymerase can effectively amplify > 20kb. For genome, the polymerase can effectively amplify > 8kb. And for cDNA, the polymerase can effectively amplify > 8kb.

  • lHigh specificity: With hot start technology, the polymerase is 100% inactive below 50°C, and can only be restored by heating at 95°C for 5min.

  • lHigh tolerance to impurities: Samples of whole blood, serum, cultured cells and urine can be directly amplified without prior DNA purification.

Unit Definition

One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol dNTP into an acid insoluble substance within 30 min at 74°C.

Components

1,000U

Tolerance to inhibitors

Recommended Sample Volume (50μl)

Storage

Minimum shelf life is 3 years at -20°C.

Instruction: Protocol

Only for research and not intended for treatment of humans or animals


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