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PrimeTaq™ HS DP DNA/RNA Polymerase


Cat. No.

Size

Price (US$)

PTHSR-250

250U

700.00

PTHSR-2500

2,500U

4000.00


For direct amplification of DNA only, please see PrimeTaq™ HotStart Direct PCR DNA Polymerase.

Features

  • lHigh DNA template dependent polymerase activity and RNA template dependent reverse transcriptase activity.

  • l5 '- 3' exonuclease activity for TaqMan probe cleavage.

  • lFor RT-PCR, the RNA template should be no longer than 280 bp. The recommended length range is 70-150 bp.

  • lThe polymerase is 100% inactive below 50°C and can be completely recovered only after heating at 92°C for 5 min.

Unit Definition

One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol dNTP into an acid insoluble substance within 30 min at 68°C.

Components

250U

Storage

Minimum shelf life is 3 years at -20°C.

Instruction: Protocol

Only for research and not intended for treatment of humans or animals


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