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We provide robust and reliable solutions for the study of various diseases (African Swine Fever, COVID-19, etc) based on SD TM DNA/RNA Polymerase and Polymerase Chain Displacement Reaction (PCDR) technoloy, please contact us or email to tech@sbsbio.com for more details.
For TaqMan PCDR of DNA templates only, please see SD TM DNA Polymerase.
Features
lStrong strand displacement activity and polymerase activity.
lStable at high temperature.
lTolerable to many PCR inhibitors such as ethanol, serum, plant polysaccharide polyphenols, etc.
lIdeal for long and complex templates amplification.
lWith hot start property, the polymerase is 100% inactive below 50°C and can be completely recovered only after heating at 90°C for 5 min.
lParticularly effective for TaqMan PCDR of both DNA and RNA templates.
Components
Unit definition
One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol dNTP into acid-insoluble substances in 30 min at 68 °C.
Storage
Minimum shelf life is 2 years at -20°C.
About PCDR
Polymerase chain displacement reaction (PCDR) uses multiple nested primers in a rapid, capped, one-tube reaction that increases the sensitivity of normal quantitative PCR (qPCR) assays. In PCDR, when extension occurs from the outer primer, it displaces the extension strand produced from the inner primer by utilizing a polymerase that has strand displacement activity. This allows a greater than 2-fold increase of amplification product for each amplification cycle and therefore increased sensitivity and speed over conventional PCR. Increased sensitivity in PCDR would be useful in nucleic acid detection for viral diagnostics.
Note
As the binding ability and cutting efficiency of the conventional TaqMan probe is very low in PCDR, we recommend our specialized probe for the reaction. Please contact us if you would like to order or learn more details.
Instruction: Protocol
Add: Room 11E, Building 2, No.2 Shangdi Information
Road, Haidian District, Beijing 100085, China
Tel: +86-10-62969345 +86-10-62969346
E-mail: info@sbsbio.com URL: http://www.sbsbio.com
ICP NO.05059350
