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For hot start version, please see SD HS DNA Polymerase.
Features
lStrong strand displacement activity and polymerase activity.
lStable at high temperature.
lTolerable to ethanol, guanidine salt, heparin, serum and plant polysaccharide polyphenols.
lIdeal for long and complex templates amplification.
Components
Unit definition
One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol dNTP into acid-insoluble substances in 30 min at 68 °C.
Storage
Minimum shelf life is 2 years at -20°C.
About PCDR
Polymerase chain displacement reaction (PCDR) uses multiple nested primers in a rapid, capped, one-tube reaction that increases the sensitivity of normal quantitative PCR (qPCR) assays. In PCDR, when extension occurs from the outer primer, it displaces the extension strand produced from the inner primer by utilizing a polymerase that has strand displacement activity. This allows a greater than 2-fold increase of amplification product for each amplification cycle and therefore increased sensitivity and speed over conventional PCR. Increased sensitivity in PCDR would be useful in nucleic acid detection for viral diagnostics.
Instruction: Protocol
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