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Poly(A) Polymerase


Cat. No.

Size

Price (US$)

PAP-100

100U

130.00

PAP-1k

1,000U

975.00


Content

100U

Unit Definition

One unit is defined as the amount of enzyme needed to polymerize 1 nmol AMP onto RNA within 10 min with ATP as substrate at 37°C and pH 7.9.

Thermal Inactivation

65°C, 20 min.

Storage

Stored at -20°C for 2 years.

Protocol

1. Set up the reaction system according to the following table.

2. After complete mixing, set the mixture at 37°C for 1 h. The end product can be used in various downstream experiments.

Note

The amount of base A added can be adjusted by changing the reaction time. At 37°C, the enzyme can add 30 base A in 30 min while 100 base A in 1 h.

Only for research and not intended for treatment of humans or animals


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