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Content
2,500U
10 × Klenow Buffer: 500 mM Tris-HCl (pH 8.0 at 25°C), 50 mM MgCl2, 10 mM DTT. The enzyme is compatible with PCR Buffer and endonuclease Buffer.
Applications
lGenerates probes using random primers
lRandom priming labeling
lSecond strand cDNA synthesis
lNot suitable for generating blunt ends
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 min at 37°C.
Thermal Inactivation
75°C, 20 min.
Storage
Stored at -20°C for 2 years.
Only for research and not intended for treatment of humans or animals
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