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Klenow Fragment (3´→5´ exo-)


Cat. No.

Size

Price (US$)

KNF-2500

2,500U

392.00

KNF-25k

25KU

3120.00


Content

2,500U

10 × Klenow Buffer: 500 mM Tris-HCl (pH 8.0 at 25°C), 50 mM MgCl2, 10 mM DTT. The enzyme is compatible with PCR Buffer and endonuclease Buffer.

Applications

  • lGenerates probes using random primers

  • lRandom priming labeling

  • lSecond strand cDNA synthesis

  • lNot suitable for generating blunt ends

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 min at 37°C.

Thermal Inactivation

75°C, 20 min.

Storage

Stored at -20°C for 2 years.

Only for research and not intended for treatment of humans or animals


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