Bsu DNA Polymerase (Large Fragment)

Content

2,500U

Applications

• lPrimer labelling

• lSecond strand cDNA synthesis

• lDNA strand displacement synthesis

• lSingle dA tailing

Thermal Inactivation

75℃, 20 min.

Unit Definition

One unit is defined as adding 10 nmol acidic refractory substance in 30 min at 37℃.

Buffer

• l1X Bsu Buffer: 50 mM NaCl, 10 mM Tris-HCl (Ph7.5), 10 mM MgCl₂, 1 mM DTT.

• lEnzyme storage buffer: 50 mM Tris-HCl (pH 8.0), 50 mM KCl, 1 mM DTT, 20% Glycerol.

Storage

Stored at -20℃ for 2 years.

Notes

• lDue to the lack of the 3’→ 5’ exonuclease activity, Bsu DNA Polymerase (Large Fragment) cannot excise 3' protruding ends and therefore not suitable for generating blunt ends.

• lAt 25℃, Bsu DNA Polymerase (Large Fragment) retains 50% activity, which is twice as high as the Klenow fragment (3´→5´ exo-) at the same temperature.