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Bsu DNA Polymerase (Large Fragment)


Cat. No.

Size

Price (US$)

BSU-2500

2,500U

652.00

BSU-25k

25KU

4332.00


Content

2,500U

Applications

  • lPrimer labelling

  • lSecond strand cDNA synthesis

  • lDNA strand displacement synthesis

  • lSingle dA tailing

Thermal Inactivation

75℃, 20 min.

Unit Definition

One unit is defined as adding 10 nmol acidic refractory substance in 30 min at 37℃.

Buffer

  • l1X Bsu Buffer: 50 mM NaCl, 10 mM Tris-HCl (Ph7.5), 10 mM MgCl2, 1 mM DTT.

  • lEnzyme storage buffer: 50 mM Tris-HCl (pH 8.0), 50 mM KCl, 1 mM DTT, 20% Glycerol.

Storage

Stored at -20℃ for 2 years.

Notes

  • lDue to the lack of the 3’→ 5’ exonuclease activity, Bsu DNA Polymerase (Large Fragment) cannot excise 3' protruding ends and therefore not suitable for generating blunt ends.

  • lAt 25℃, Bsu DNA Polymerase (Large Fragment) retains 50% activity, which is twice as high as the Klenow fragment (3´→5´ exo-) at the same temperature.

Only for research and not intended for treatment of humans or animals


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