Home > Products > PCR-Related > P29P PCR-Related

phi29 DNA Polymerase


Cat. No.

Size

Price (US$)

P29P-1k

1,000U

156.00

P29P-20k

20 KU

1560.00


For updated version which can continuously synthesize DNA at 42°C, please see
phi29 HT DNA Polymerase.

Content

1,000U

Features

  • lStrong strand displacement capability

  • lContinuous synthesis capability

  • lHigh fidelity of amplification

Applications

  • lDNA isothermal rolling loop amplification

  • lDNA synthesis requiring strong strand displacement

  • lRapid replication of plasmids

  • lRapid replication requiring high fidelity

Storage

Stored at -20℃ for 3 years.

Notes

  • l1 x phi29 Buffer: 50 mM Tris-HCl pH 7.5, 10 mM MgCl2, 10 mM (NH4) 2SO4, 4 mM DTT. If necessary, the final concentration of 1 mM DTT and 0.2 mg/ml BSA can be added separately to improve the reaction efficiency.

  • lThe optimum reaction temperature of the enzyme is 30℃.

  • lThe enzyme can be inactivated at 65℃ in 10 min.

  • lAdjust the concentration of dNTP from 100 to 500 μM according to the type of experiments.

  • lAddition of Yeast Pyrophosphatase to the reaction mixture with phi29 DNA Polymerase may enhance DNA synthesis.

  • lThio-modification at the 3' end of the primers can avoid primer degradation.

Note: All product outward appearance, the size color take the material object as, the picture only supply the reference.

Only for research and not intended for treatment of humans or animals


HOME    |    About US    |    NEWS    |    Products    |    Support    |    Promotion    |    Distributor    |    Order