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Features
The whole blood is directly used as a PCR template, without time-consuming and expensive DNA purification and pretreatment.
The system has strong amplification ability and can sensitively detect genomic and exogenous DNA fragments in the blood.
The 2× Scarlet™ Blood Direct PCR Mix (EDTA) provided by this kit has strong inhibitor tolerance.
The sample is operated in a fully enclosed condition, preventing either sample contamination or false positive PCR results.
The Scarlet™ Blood Direct PCR Kit (EDTA) is specially designed for direct PCR identification of EDTA anticoagulant whole blood.
Amplified fragments should be less than 1 kb. If more than 1 kb, the amplification efficiency may decrease or the amplification may even fail.
If PCR products are used for sequencing, we recommend purifying the PCR products first.
PCR products may have mutation.
The 3' end of the PCR product is randomly added with A tail.
If used frequently, 2 × Scarlet™ Blood Direct PCR Mix (EDTA) can be stored at 4°C for a short time (within 10 days). For long term storage, please keep it at - 20°C.
6 × DNA Loading Buffer can be stored at 4°C or - 20°C for a long time.
Instruction: Protocol
Add: Room 11E, Building 2, No.2 Shangdi Information
Road, Haidian District, Beijing 100085, China
Tel: +86-10-62969345 +86-10-62969346
E-mail: info@sbsbio.com URL: http://www.sbsbio.com
ICP NO.05059350
