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SuperGold™ High Fidelity PCR EasyMasterMix (5 x 1ml)


Cat. No.

Size

Price (US$)

SGEMM-5

5×1ml

98.00


Description

This mix contains SuperGold™ High Fidelity DNA polymerase, dNTPs, MgCl2, and buffer. Among them, SuperGold™ High Fidelity DNA polymerase has extremely high fidelity, which is 69 times as high as Taq DNA polymerase. Extremely high fidelity makes it possible to clone ultra-long fragments. And simple templates such as plasmids and lambda DNA can be amplified by SuperGold™ High Fidelity PCR MasterMix with an effective length of 40 kb, genomes can be amplified with an effective length of 20 kb, and difficult fragments such as cDNA can be amplified with an effective length of 10 kb. In addition, the extension rate of SuperGold™ High Fidelity DNA polymerase can reach as fast as 15 sec/kb, which is four times as high as Taq DNA polymerase.

By using 1 x SuperGold™ High Fidelity PCR MasterMix, PCR reaction can be performed by simply adding only primers and templates, which reduces pipetting operations and maximizes throughput and repeatability of results. The EasyMasterMix itself contains blue-purple dyes, which can be directly sampled for gel electrophoresis after amplification.

Instruction: Protocol

Only for research and not intended for treatment of humans or animals


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