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Unit Definition
One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nanomoles of dNTPs into acid insoluble material in 30 minutes at 74°C.
Components
250U
Pfu DNA Polymerase Storage Buffer: 20mM Tris-HCl (pH8.0), 0.1 mM EDTA, 1mM DTT, Stabilizers, 50% glycerol.
10 x Pfu Buffer: 200 mM Tris-HCl (pH8.8), 100 mM KCl, 100 mM (NH4)2SO4, 20 mM MgSO4, etc.
Instruction: Protocol
Only for research and not intended for treatment of humans or animals
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