Ultra Thermo-stable M-MLV Reverse Transcriptase (RNase H-)

Description

Ultra Thermo-stable M-MLV Reverse Transcriptase (RNase H-) is a mutant type of M-MLV Reverse Transcriptase, obtained by eliminating the active center of RNase H through multiple point mutations. The alteration decreases activity of RNase H and reduces RNA degradation in reverse transcription, which increases the yield of first-strand cDNA to get full length cDNA more easily. In addition, thermal stability of the reverse transcriptase is improved and the enzyme is active up to 65°C.

Source and Application

The enzyme is obtained from E.coli strain containing mutant Molony murine leukemia virus (M-MLV) reverse transcriptase gene. It could be applied in first-strand cDNA synthesis and RT-PCR.

Storage

Minimum shelf life is 2 years at -20°C.

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Instruction: Protocol

Only for research and not intended for treatment of humans or animals