PreScission Protease (PSP)

Description

PreScission Protease specifically recognizes Gln and Gly residues in the short peptide Leu-Glu-Val-Leu-Phe-Gln/Gly-Pro for cleavage. The recognition and cleavage of substrates depend not only on the primary structure of the fusion protein but also on the secondary and tertiary structure. It can specifically separate GST tags with enzyme substrate recognition polypeptide sequence fusion proteins expressed by vectors such as pGEX-6P series. The source of this protein is the recombinant expression of E. coli.

Unite definition

The amount of enzyme required to cleave more than 90% of 100 μg GST-tagged fusion protein after 16 hours of reaction at 5 °C is defined as an active unit.

Storage

PreScission Protease can be stored for 2 years at -80 °C for a long time or for 6 months at -20 °C after sub-packaged to avoid repeated freezing and thawing. 10 x PreScission Buffer can be stored at -20 °C.

PSP Stock Solution：25mM Tris-HCl, pH 8.0, 150mM NaCl, 1mM EDTA, 5mM DTT, 50% Glycerin

10× PreScission Buffer: 500mM Tris-HCl, pH 7.0, 1.5M NaCl, 10mM EDTA, 10mM DTT

Only for research and not intended for treatment of humans or animals