LbaCas12a (Cpf1)

Description

LbaCas12a (Cpf1) has a RuvC endonuclease domain similar to Cas9 but does not have the HNH endonuclease domain. When editing, LbaCas12a does not need tracrRNA. Only crRNA is required. This is very helpful for genome editing because LbaCas12a is not only smaller than Cas9, but also has a smaller crRNA molecule (nearly half of Cas9's total sgRNA). In contrast to Cas9 which needs G-rich PAM sequence, LbaCas12a crRNA complex cleaves target DNA by recognizing T-rich PAM motifs (TTTN). The double strand breaks of DNA after LbaCas12a cleavage introduce four or five nucleotide protrudent sticky ends.

When LbaCas12a recognizes and cleaves the target double-stranded DNA under the guidance of guide RNA, its "accessory cleavage" activity is activated, which can efficiently cleave non-specific single-stranded DNA (ssDNA) in the reaction system. By designing ssDNA probes with fluorescent groups or other markers at both ends, CRISPR/Cas12b can detect DNA template and amplify the signal, to realize the detection of target. The system has high sensitivity and strong specificity.

Content

Applications

• lReal-time Fluorescent Detection

• lColloidal gold chromatography

• lGene editing

• lMolecular diagnosis

Storage