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Description
When AapCas12b recognizes and cleaves the target double-stranded DNA under the guidance of guide RNA, its "accessory cleavage" activity is activated, which can efficiently cleave non-specific single-stranded DNA (ssDNA) in the reaction system. By designing ssDNA probes with fluorescent groups or other markers at both ends, CRISPR/Cas12b can detect DNA template and amplify the signal, to realize the detection of target. The system has high sensitivity and strong specificity.
Content
Applications
lReal-time Fluorescent Detection
lColloidal gold chromatography
lGene editing
lMolecular diagnosis
Storage
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