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Peptide Library General Information


Peptide library applications

A peptide library is a new technique for studying structure-function relationship for a protein. A peptide library contains a great number of peptides that have a systematic combination of amino acids. The peptide library provides a powerful tool for drug design, protein-protein interactions, and other biochemical as well as pharmaceutical applications. It also has wide applications in drug screening, target validation, epitope mapping, and vaccine development. Some other applications of peptide library are:

  • Receptor (GPCR) ligand screening

  • Peptide Drug discovery

  • Protein-Protein interaction

  • Functional proteome

  • Peptide-Nucleic acid binding

  • Enzyme substrate or Peptide-Inhibitor screening

  • Antigen binding sites or epitope mapping

  • T-Cell epitope disovery

  • Antoantibody binding sites on antigens (protein)

  • Antibody cross reaction with a related protein family member

  • Identification of small peptides as vaccine candidates


Peptide library specifications

At BeijingSBS Genetech, peptide library is usually supplied as either desalt or >70%, >80%, >90%, and >95% purities). Peptides can be made in required amounts from minimal to mg scale for initial screening. Appropriate peptide labels (Biotin, FITC, etc.) can be added upon request. Each peptide in the library undergoes rigorous quality control to avoid any cross contaminants before delivery. All purified peptides are delivered with complete QC data including RP-HPLC, MS, and COA report to ensure high quality. The desalted peptides are provided with MS only. Peptide length in a given library is usually 5-15 aa with 1-5 amino acid overlap.


Peptide library types

Depending upon a given application, peptide libraries are usually defined as:

 

A. Overlapping Peptide Library


Overlapping peptide library can be used for epitope mapping. The peptide length (5-15aa) and offset number or the degree of overlapping amino acids (2-5 aa) is determined by the length of the full length proteins, overall experiment objectives and the cost. As a general guideline, shorter peptides have less chance for multiple epitope hits. Greater degree of overlapping (small offset number) gives more chances of multiple epitope hits.

Example:   (peptide length 10 aa, offset/overlap 3 aa)


       DSTRKLMNRT QSEDKLPSRG QHTRSDKENN PSSTRGHLED (30 aa protein)

 

       DSTRKLMNRT                                     Peptide #1

            RKLMNRTQSE                             Peptide #2

                  MNRTQSEDKL                     Peptide #3

                        TQSEDKLPSR             Peptide #4


Total peptides, n=11

 

B. Alanine Scanning Library

Example:   (peptide length 10 aa)

       DSTRKLMNRT                                     Peptide #1

       ASTRKLMNRT                                      Peptide #2

       DATRKLMNRT                                     Peptide #3

       DSARKLMNRT                                    Peptide #4

       DSTAKLMNRT                                     Peptide #5

       DSTRALMNRT                                     Peptide #6

       DSTRKAMNRT                                    Peptide #7

       DSTRKLANRT                                      Peptide #8

       DSTRKLMART                                     Peptide #9

       DSTRKLMNAT                                     Peptide #10

       DSTRKLMNRA                                    Peptide #11

 

C. Truncation Library


Truncated peptide library can be used to identify the shortest amino acid sequence needed for a given function or activity.  


Example:   (peptide length 10 aa) Truncation from the N-terminus


       DSTRKLMNRT                              Peptide #1 (control peptide with full function)

         STRKLMNRT                              Peptide #2

          TRKLMNRT                              Peptide #3

            RKLMNRT                             Peptide #4

              KLMNRT                             Peptide #5

                LMNRT                             Peptide #6

                  MNRT                            Peptide #7


 Truncated peptides can also be generated from the C-Terminus.

 

D. Random/scrambled Library


Scramble Library is constructed by carrying out permutation on the original peptide’s sequence. The number of each amino acid (e.g. 2 Arg or R) is the same in the peptide except its position and other amino acids are created at random. This strategy is used for preliminary identification of a group of active sequences that may possess a given function or activity.


       DSTRKLMNRT              Peptide #1 (control peptide with full function)

       TRLKSTNRMD              peptide # 2

       RSTRLDMNKD             peptide # 3  etc

 

E. Positional Scanning Library


A selected position or positions in a peptide sequence are each systematically replaced with all other natural amino acids (20) or any other amino acid to determine the preferred amino acid residues at a given position that will maximize the peptide activity.


Example:   (peptide length 10 aa, with substitution of “K at position 5.

       DSTRKLMNRT                      Peptide #1 (control peptide)

       DSTRALMNRT                      Peptide #2

       DSTRCLMNRT                      Peptide #3

       DSTRDLMNRT                      Peptide #4

       DSTRELMNRT                      Peptide #5

       DSTRFLMNRT                      Peptide #6

       DSTRGLMNRT                      Peptide #7


Potential peptides =20 with all 20 natural amino acids. It is also possible to study the replacement of “N” at position #8.

 

Peptide Library Pricing

Peptide libraries are synthesized on SBS Genetech’s unique proprietary synthesis platform and are typically supplied at the 2-4 mg scale for fast, efficient screening work.  


Peptide library pricing (per peptide) for up to 15 amino acids.
Library Size48-96 Peptides97-200 Peptides201-300 Peptides301-400 Peptides>400 Peptides
Desalt$32$29$27$24$23
>70%$62$57$53$51$49
>80%$91$83$79$75$70
>90%$113$103$98$92$88
>95%$123$111$105$103$95

◎Desalted peptides are provided with MS only.

◎Purified peptides are provided with MS and HPLC data.


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