U-Taq DNA Polymerase

U-Taq DNA Polymerase is a thermostable enzyme that can withstand prolonged incubation at temperature up to 95℃ without significant loss of activity. The enzyme consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5'→3' DNA polymerase activity and lacks 3'→5' exonuclease activity. Its extension rate is 2~4 kb/min in standard condition, which is the highest among all thermostable DNA enzyme. The appropriate reaction temperature is 70~75℃, the work concentration of dNTPs is 100~300 μM, the work concentration of Mg²⁺ is 2~3 mM, and the suitable pH is 8.1~9.1. The enzyme generates PCR products with 3'-dA overhangs, suitable for T-A cloning. The amount of enzyme is 1~1.5unit for 20μl PCR reaction, while 2~3unit for 50μl PCR reaction. 6 kb Lambda DNA and 2.1 kb Human genomic DNA can be amplified very well at our laboratory.

Protocol.pdf