First-Strand cDNA Synthesis Kit


The Kit is a complete system for efficient first-strand cDNA synthesis from RNA templates. First-Strand cDNA Synthesis Kit uses M-MLV Reverse Transcriptase, which exhibits lower RNaseH activity compared to AMV Reverse Transcriptase. The Kit contains oligo(dT)18 and random nonamer primers (dN)9. Oligo(dT)18 is designed to prime selectively on mRNA with a poly(A)+ tail. A random nonamer can be used for the generation of cDNA libraries, to copy mRNAs that do not possess a poly(A)+ tail, or for synthesis of the 5'-end regions of mRNAs. The cDNA synthesized using this system can be used directly in PCR amplification.


Cat. No.SizePrice (US$)
FFS-2525 rxn53.00


Protocol.doc


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