Two-Step RT-PCR Kit


  The Two-Step RT-PCR Kit has all the components required to perform first-strand cDNA synthesis followed by amplification of the cDNA product using a two-step process. In the first step, M-MLV Reverse Transcriptase is used to synthesize a DNA copy of input RNA. M-MLV RTase is a frequent choice for cDNA synthesis because of its ease of use and low intrinsic RNase activity. Oligo(dT)18 primers direct synthesis of a complementary strand of poly(A)+ mRNA. Random nonamer primers direct synthesis of sequences complementary to all RNA present in the reaction.

In the PCR step, a portion of the cDNA product is amplified using U-Taq DNA polymerase and input primers. U-Taq polymerase, buffer, dNTPs are included in this kit.

Key Features:

Optimized for high performance using M-MLV Reverse Transcriptase and Taq DNA polymerase.

Choice of priming methods: oligo(dT)18, random nonamer or gene-specific primers.

Amplifies targets up to 1.5 kb very well at our laboratory.


Cat. No.SizePrice (US$)
FTRP-25 25 rxn92.00



Protocol.doc


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